In univariate analysis, RFS was significantly associated with tumor diameter (HR [hazard ratio]=2.512, 95% CI=1.562–4.040, P<0.001), tumor number (HR=1.863, 95% CI=1.033–3.361, P=0.039), microvascular invasion (HR=2.477, 95% CI=1.551–3.956, P<0.001), serum AFP (HR=2.115, 95% CI=1.269–3.526, P=0.004) and serum SP70 (HR=2.008, 95% CI=1.247–3.233, P=0.004). The gene discussed is AFP; the disease is neoplasm.