HK2 and migraine disorder: Compared with CON group, quantitative real-time polymerase chain reaction showed that PFKP (2.02 ± 0.51), HK2 (2.55 ± 0.72), HIF-1α (3.05 ± 0.61) and PKM2 (1.97 ± 0.49) mRNA expression increased in migraine model group (Figures 1A–E).