PRF1 and neoplasm: Importantly, RNA sequencing data of entinostat-treated human NK cells before co-culture showed transcriptional changes, revealing upregulation of molecules related to NK cell cytotoxic functions, such as GZMB, PRF1, IFNG, and TNFA. Thus, HDAC inhibition in NK cells led to significant transcriptional profile alterations, independently of NK cell ligand modulation or co-culture with tumor cells (127).