As we noted a marked heterogeneity in the CD4 and CD8 abundance between ROIs in samples where multiple ROIs were analysed (supplemental fig 6I), we assume that the moderate correlation between RNA-Seq and IMC data at least partially resulted from comparing IMC data from small 1-2mm2 large ROIs to RNA-derived immune deconvolution from entire tumour sections. This evidence concerns the gene CD4 and neoplasm.