The results of behavioral tests indicated that this treatment eliminated the depression-like behaviors in Sig-1R knockout mice, as evidenced by the lack of significant differences in latency to immobility, immobility time, and sucrose preference index between the WT + ABX and KO + ABX groups in the TST, FST, and SPT experiments (Figures 1B–F). This evidence concerns the gene SIGMAR1 and depressive symptom measurement.