The expression levels of SPP1, CXCL8, and THBS1 were higher in NASH-HCC tissues than those in NASH tissues, while the expression levels of ESR1, CAT, CD4, CYP2E1, CYP3A4, UGT2B7, and GSTA1 were lower in NASH-HCC tissues than those in NASH tissues. This evidence concerns the gene SPP1 and metabolic dysfunction-associated steatohepatitis.