These results are also reflected at the transcriptome level where PCK1 was the most upregulated gene (S1 Table 2) and significant gene expression changes in relevant pathways (insulin resistance (adjusted p = 0.04), insulin signalling pathway (adjusted p = 0.02) and glycolysis/gluconeogenesis (adjusted p = 3 × 10−4)) were observed (Fig. 3g and S1 Table 3). Here, PCK1 is linked to Insulin resistance.