To confirm this result, we performed RT-qPCR and immunoblotting (IB) analyses and found that ablation of UTP11 by two independent siRNAs markedly increases the expression of p53 and p21 at protein levels as well as several target genes at RNA levels, such as p21, BTG2, and MDM2, in both CAL-51 and MCF-7 breast cancer cell lines (Fig. 1D–G). The gene discussed is MDM2; the disease is breast cancer.