In A549 human lung cancer cells, maintained in growth medium supplemented with 100 nM sodium selenite for optimal selenoprotein saturation and TXNRD1 activity [33], a clear dose-dependent suppression in RX1 signal was seen with RSL3 and ML162 concentrations of 0.5 μM or higher, but not with ML210 (Fig. 2A and B). This evidence concerns the gene TXNRD1 and lung cancer.