SELENOS and lung carcinoma: In A549 human lung cancer cells, maintained in growth medium supplemented with 100 nM sodium selenite for optimal selenoprotein saturation and TXNRD1 activity [33], a clear dose-dependent suppression in RX1 signal was seen with RSL3 and ML162 concentrations of 0.5 μM or higher, but not with ML210 (Fig. 2A and B).