Western blot analysis revealed a marked increase in nSREBP2, but no significant change in nSREBP1 in CRC cells overexpressing circINSIG1 or circINSIG1-121, suggesting that circINSIG1-121 might promote SREBP2 activation to enhance cholesterol biosynthesis (Fig. 4G). This evidence concerns the gene SREBF2 and colorectal carcinoma.