Since RNA‐seq showed that the key angiogenic factor, VEGF‐A, was downregulated in HOOK1 overexpression cells compared with control cells (Figure 5A), we then explored whether HOOK1 mediated RCC angiogenesis by inhibiting VEGF‐A via TGF‐β signal. The gene discussed is TGFB1; the disease is renal cell carcinoma.