Similarly, although the RCC culture medium overexpressing ALK5 promoted HUVECs’ migration and/or tube formation to some extent, ALK5 did not eliminate the angiogenic inhibitory effect of HOOK1 (Figure S5B,C, Supporting Information), suggesting that HOOK1 might inhibit RCC angiogenesis through other mechanisms. The gene discussed is HOOK1; the disease is renal cell carcinoma.