To further confirm that Roblitinib and CPI-169 synergistically induce HCC cell apoptosis depends on YAP signaling, we first took advantage of a luciferase reporter gene system to examine the YAP/TAZ activity of HepG2 cells, SMMC-7721 cells and cells expressing YAP1S127A following the combination treatment. The gene discussed is YAP1; the disease is hepatocellular carcinoma.