Immunofluorescence assay was used to detect the fluorescence intensity of LC3 to evaluate autophagy, and results indicated that exposure to AMSC-Exo-342 potentiated the fluorescence intensity of LC3 (Fig. 6A, P < 0.01), and AMSC-Exo-342 + si-TLR9 treatment further increased the fluorescence intensity of LC3 (Fig. 6A, P < 0.05); however, overexpression of TLR9 weakened the effects of AMSC-Exo-342 on autophagy in sepsis cellular models (Fig. 6A, P < 0.05). Here, TLR9 is linked to Sepsis.