Therefore, to target key components of the Rho circuitry by using different strategies (in red, Fig. 1A), we purposely mined databases of gene expression (GTEx and TCGA) comparing glioblastoma and low-grade glioma (LGG) tumors with normal tissues (Fig. 1B) and found that the RHOA, RHOC, DIAPH1, PFN1 and CFL1 genes were more highly expressed in GBM, while RHOB and MYPT1 were more highly expressed in LGG. This evidence concerns the gene PPP1R12A and central nervous system cancer.