The present results reveal that CX3CL1 induces the migration of OSCC cells by promoting ICAM‐1 expression through the CX3CR1 and the PLCβ/PKCα/c‐Src signal pathway, suggesting that CX3CL1‐CX3CR1‐mediated signalling is correlated with tumour motility and appealed to be a precursor for prognosis in human OSCC. This evidence concerns the gene CX3CL1 and neoplasm.