Western blot and immunofluorescence showed that the expression of ZO-1 and occludin proteins was decreased in ARDS mice, and that the SIRT6 inhibitor OSS_128167 further aggravated the downregulation of ZO-1 and occludin proteins in LPS-induced ARDS mice (Figure 6B and C). The gene discussed is TJP1; the disease is acute respiratory distress syndrome.