To clarify the role of SIRT6-mediated autophagy and further explore whether SIRT6 protects against ARDS through ERK1/2 signaling, we used the ERK1/2 inhibitor PD98059, si-SIRT6, and the autophagy agonist rapamycin on A549 cells. The gene discussed is MAPK3; the disease is acute respiratory distress syndrome.