RNLS and renal fibrosis: Compared with the UUO+Ad-Renalase group, after overexpression of GSK-3β in the UUO+Ad-Renalase+AAV-GSK-3β group, the anti-renal fibrosis effect of Renalase was almost reversed (as mentioned above; Figure 2C, D, E), while the expression of ER stress markers was still significantly increased (Figure 3D, E).