Western blotting showed that the levels of Col and FN were slightly decreased (Figure 3E), but the difference was not statistically significant, indicating that compared with the UUO+Ad-Renalase group, knockdown of GSK-3β did not further improve renal fibrosis, which further indicated that the anti-fibrosis effect of Renalase was through inhibition of GSK-3β/Snail. The gene discussed is RNLS; the disease is renal fibrosis.