Dithizone- or Diphtheria toxin-induced PC depletion. Model to study role of PCs in NEC, intestinal development closer to human development, model further simplified with either exposure to hyperosmotic formula (RMS) or bacterial challenge (Klebsiella, NECteria); short protocol time, TLR4-independent pathway. The gene discussed is TLR4; the disease is necrotizing enterocolitis.