The gold standard for the diagnosis of HSCR is rectal mucosal aspiration biopsy and pathological diagnosis, which are commonly invasive and difficult to perform.1 2 The relative expression of the key TRN regulons (has-miR-107, has-miR-10b-5p, has-miR-659-3p, has-miR-371a-5p, PIM3, CHUK, F2RL1, and CA1) were visualized as boxplots (figure 8A, B). This evidence concerns the gene F2RL1 and Hirschsprung disease.