We utilized EA and AA nonmalignant prostate and prostate cancer cell models with confirmed genomic ancestry and defined the basal and 1α,25(OH)2D3-regulated VDR protein interactome (RIME), the VDR cistrome [Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq) and chromatin immunoprecipitation sequencing (ChIP-seq)], and the VDR transcriptome [RNA sequencing (RNA-seq)]. This evidence concerns the gene VDR and Familial prostate cancer.