Notably, after incubating the WT and mutant GCK in a 37°C water bath (simulating normal body temperature in humans) for 30 min, the activity of mutant GCK was found to be much lower than that of WT GCK, suggesting that a decrease in GCK activity in vivo might result in the disruption of the phosphorylation of glucose to glucose 6-phosphate process, and may result in glucose metabolism disorders. The gene discussed is GCK; the disease is glucose metabolism disease.