Transwell assay images showed the migration and invasion of negative control group, FOXD3-AS1 knockdown group, FOXD3-AS1 overexpression group and FOXD3-AS1 rescue group, as well as quantitative analysis of glioma cell migration and invasion, indicating that interference with FOXD3-AS1 expression could promote the migration and invasion of glioma cells in vitro to some extent, and we found that the migration and invasion of U87 and U251 cell lines were enhanced after overexpression of FOXD3-AS1 (Figures 15F–K). The gene discussed is FOXD3; the disease is glioma.