Through the new method, we have successfully detected corresponding ADGRG6 enhancer fragments in urine samples from bladder cancer patients, healthy individuals and urinary tract infection or urinary stones patients, the result revealed that the ARMS‐qPCR assay's specificity is 98.4% and sensitivity is 83.3%, which would be a good test for genomic mutations screening and contribute to bladder cancer diagnosis. Here, ADGRG6 is linked to urinary bladder carcinoma.