In the present study, according to other researchers' experiences with excellent screening methods for bioinformatics of LUAD,35 higher STK4 expression was observed to be positively related to the prolong OS, lower STK4 expression was observed in tumor tissues compared with normal tissues, we also proved the interaction of miR‐28‐5p‐STK4 and G‐Rh2‐STK4 in NSCLC for the first time. Here, STK4 is linked to neoplasm.