As CD103+ Tregs are potent suppressors of CD8+ T cell responses (Fig. 4k), we next examined both CD8+ T cell content and their activation state in RT + Conc.IT + aCD25-treated glioblastoma and observed an accumulation of CD8+ T cells with elevated GrzA expression (Fig. 6c,d), confirming the results obtained in ex vivo Treg suppression assays. This evidence concerns the gene CD8A and glioblastoma.