We further explored the functional differences between CD103+ and CD25+ Treg populations by performing ex vivo suppression assays60 of CD8+ T cells co-cultured with CD25− T cells, CD25+ or CD103+ Tregs isolated from d12 RT + Conc.IT-treated glioblastoma (Extended Data Fig. 6a). The gene discussed is ITGAE; the disease is glioblastoma.