We applied ssGSEA for the immune infiltration analysis and found that 16 immune cell types exhibited significant differences in distribution between AIS and control groups, including activated CD8 T cell, central memory CD8 T cell, effector memory CD8 T cell, activated CD4 T cell, gamma delta T cell, regulatory T cell, activated B cell, memory B cell, natural killer cell, CD56dim natural killer cell, activated dendritic cell, plasmacytoid dendritic cell, macrophage, mast cell, monocyte and neutrophil. Here, CD4 is linked to androgen insensitivity syndrome.