We have shown that RBM4 reduction restricted ESCC cellular consumption of glutamine, which is a versatile nutrient that participates in biomass, including purine and pyrimidine synthesis, energy production and redox homeostasis.67 Therefore, we examined the tentative effect of altered glutamine metabolism in RBM4-reduction induced cellular senescence and proliferative arrest. This evidence concerns the gene RBM4 and esophageal squamous cell carcinoma.