To analyze the role of GR in macrophages in obesity-induced insulin resistance, we placed wild-type (GRflox) mice and mice with a reduced GR expression in the monocyte/myeloid lineage, including BMDMs, ATM, and Kupffer cells (Supplementary Fig. S1G), Nr3c1tmGsc; Lyz2tm19(cre)ifo (hereby referred to as GRLysMCre), on a high-fat diet (HFD) or chow diet. Here, NR3C1 is linked to obesity due to melanocortin 4 receptor deficiency.