Functional over-representation analysis revealed that proteins increased in ALS were enriched in protein metabolism (e.g. PSDMD9, NAE1, PSMB5), RNA metabolism (e.g. APP, CSTF1, CIRBP, SNRPD2), protein binding (e.g. RRBP1, RPS29, EIF1), as well as other processes established in ALS pathophysiology (stress response, cholesterol synthesis, nucleocytoplasmic transport) whilst proteins decreased in ALS, were enriched in Golgi transport (Supplementary Fig. 11b). The gene discussed is PSMB5; the disease is amyotrophic lateral sclerosis.