Observing the other TF activity changes between genetic backgrounds revealed 5 TFs that were significantly changed in the same direction across 4 of 5 genetic backgrounds, of which ZNF274 was increased whilst GATA3, MAZ, TAL1 and TEAD4 were decreased in the ALS subgroups (Supplementary Fig. 13b). The gene discussed is TEAD4; the disease is amyotrophic lateral sclerosis.