Flow cytometry analysis to detect apoptosis revealed that Na2EA induced 60–75% cellular apoptosis in three AML cell lines (Fig. 4A and S7D-S7I); ME2 inhibition by Na2EA treatment also decreased NADPH production (Fig. 4B), which resulted in ROS accumulation in the cell (Fig. 4C), indicating that ME2 inhibition disrupted ROS homeostasis and was therefore detrimental to AML cell growth. This evidence concerns the gene ME2 and acute myeloid leukemia.