Similar to ME2-silenced cells, treatment with Na2EA inhibited ME2 activity in HL-60, THP-1, and MV4-11 AML cell lines, resulting in a decrease in pyruvate and NADH levels and an increase in the NAD+/NADH ratio (Fig. 4 M, 4 N and 4O, respectively), indicating that ME2 inhibition is detrimental to energy metabolism, which corresponds cellular respiration and ATP synthesis. The gene discussed is ME2; the disease is acute myeloid leukemia.