We previously reported that cMyc acetylation at K148 is required for the recruitment of AF9, which is a subunit of the super elongation complex and exacerbates proliferation in liver cancer cells.[27] Herein, we examined the formation of the PHGDH/p300/cMyc/AF9 axis in the cells expressing WT or K148R cMyc. The gene discussed is MLLT3; the disease is liver cancer.