We then examined the soluble levels of CXCL1, IL8, and IL1B in the medium of cultured PLC/PRF/5 or Hep3B cells expressing WT or dACT and found that dACT reduced the concentration of secreted CXCL1, IL8, and IL1B in the medium, suggesting the decreased expression of CXCL1, IL8, and IL1B in liver cancer cells (Figure 5F and Figure S5B, Supporting Information). This evidence concerns the gene IL1B and liver cancer.