Next, we detected Treg cells, Th subsets in CD4+ T cells and Tc subsets in CD8+ T cells, the results showed the frequency of Th2 cells in the circulation of patients with HBV-HCC and HBV-LC was significantly higher than that in healthy volunteers (p<0.05, p<0.05), while there was no significant difference in the frequency of Th2 cells between patients with HBV-HCC and patients with HBV-LC (Figure 2C). This evidence concerns the gene CD8A and hepatocellular carcinoma.