To determine whether peripheral circulating lymphocytes are associated with diagnosis of HBV-HCC, we plotted ROC curve for the frequency of lymphocytes which was different between healthy controls and HBV-HCC patients, the results demonstrated that the combination of CD3+ T cell and CD8+HLADR+CD38+ T cell may be a potential diagnostic indicator for HBV-HCC. Here, CD38 is linked to hepatocellular carcinoma.