Similarly, cluster analysis of CD8+ T cells showed that the frequency of terminally differentiated effector CD8+ T (TEMRA) cells in the circulation of HBV-HCC patients was significantly higher than that of healthy volunteers (P <0.05) and HBV-LC patients (p <0.01) (Figure 2B; Supplementary Figure 1B). This evidence concerns the gene CD8A and hepatocellular carcinoma.