Two main subsets of γδ T cells in human peripheral blood, Vδ1 and Vγ9Vδ2, we found that the expression of PD-1 on Vδ1 cells was increased in HBV-HCC patients, and we found that TIM3+ γδ T in peripheral blood of patients with advanced HBV-HCC was higher than that in early stage, suggesting that the expression of TIM3 on γδ T cells may be related to disease progression. The gene discussed is PDCD1; the disease is hepatocellular carcinoma.