According to the expression of CD45RA and CCR7, the analysis of CD4+ T cells and CD8+ T cell subsets showed that the frequency of TEM in CD8+ T cells of patients with advanced HCC was significantly lower than that of patients with early HCC, and there was no significant difference in the frequency of other CD4+ T cell subsets between the two groups (Figures 8B–D). This evidence concerns the gene CD4 and hepatocellular carcinoma.