To examine whether BCAAs stimulate cell growth and invasion via mammalian target of rapamycin (mTOR) signalling in ICC, we examined the expression of the key catalytic enzymes of BCAAs, namely BCAT1 and BCAT2, in human ICC cell lines (SSP-25, RBE, and HCCC-9810) in comparison with their expression in normal human dermal fibroblast cells. This evidence concerns the gene BCAT1 and intrahepatic cholangiocarcinoma.