The frequencies of immune cells (CD4+ T cells, CD8+ T cell, Treg cells, NK cells and CTLs) and the concentrations of cytokines (including IL-6, IL-12, Interferon (IFN)-γ, IL-10 and TGF-β) in tumour tissues were used to evaluate the immune status of the tumour microenvironment. The gene discussed is TGFB1; the disease is neoplasm.