Based on charge distribution plots (Fig. 1A), we hypothesized that phosphomimetic substitutions within the Pro-rich domain (PM PRD) (19 phosphorylation sites have been identified in this region in AD (13, 14, 15)) will have a deleterious effect on LLPS, as these substitutions reverse the overall charge in the middle region of tau from positive to negative, diminishing the potential for attractive intermolecular electrostatic interactions (Fig. 1A and Table 1). The gene discussed is MAPT; the disease is Alzheimer disease.