Finally, CRISPR depletion of RUVBL1 significantly retarded the EwS tumor progression (Figure 1J; sgCtrl = 423.5 ± 44.5 mm3; sgRUVBL1 = 77.9 ± 18.1 mm3; data represent day 15 mean tumor volume ± SEM) with a drastic induction of cleaved caspase 3 staining (Figure 1K; an apoptotic marker) in the A673 xenograft model, indicating the indispensable role of RUVBL1 in EwS maintenance. Here, RUVBL1 is linked to neoplasm.