Genes with two or more hairpins that were > twofold enriched on day 14 acini culture compared to day zero were identified (Table 1, Table S1), and the selection of genes (i.e. COMMD3, Evi2A, ACVR1B, EphA10, AXL, KHK, ITK, ARHGAP28, DLG2) for further validation was based on literature searches for their association with cancer progression and selected genes were tested in a single hairpin per well approach. Here, KHK is linked to cancer.