The video of the NTA is shown in Additional File 5 Moreover, as shown in Fig. 5c, all sEV samples obtained from the plasma of both CRC patients (P1-5) and healthy subjects (HS1-5) were characterized for the presence of EV markers (CD81 and Alix) and for the absence of proteins expected to be underrepresented in EVs (Calnexin and Cytochrome C). The gene discussed is CYCS; the disease is colorectal carcinoma.