C4B and obesity due to melanocortin 4 receptor deficiency: These data suggest that, among the enrichment of many pathways (Supplemental Figure 1K), loss of Tet2 in pre-LHSCs/PCs under conditions of obesity induced higher intracellular levels of Ca2+, which was associated with reduced 5-hmC levels and exacerbated CH compared with control HSC/Ps.