In this study, we transfected a specific lentivirus-mediated short hairpin RNA (shRNA) into pancreatic cells and studied the effect of PROK1 silencing on PC in vivo by monitoring the growth of the tumor, determining cell apoptosis, detecting the protein level of proliferation markers and anti-apoptotic markers, and measuring the activation level of the key molecules of the PI3K/AKT/mTOR signal. Here, AKT1 is linked to pachyonychia congenita.