We verified via Co-IP and WB analysis that TM4SF1 could pull down MYH9, Meanwhile, WB experiment also verified that as a downstream molecule, M MYH9 is positively regulated by TM4SF1 and MYH9 expression in HCC cells was favorably connected with the NOTCH pathway's associated components. The gene discussed is TM4SF1; the disease is hepatocellular carcinoma.