We further showed that the use of NovB2 and Tax during lentivirus vector production (Extended Data Fig. 6a) had no impact on transduction efficiency (Extended Data Fig. 6b), the cytolytic capacity of CAR-T cells against target cells (Extended Data Fig. 6c), the levels of inducibly expressed gene cargo upon CAR-T cell co-culture with target cells (Extended Data Figs 6d,f) or tumour control by anti-CD19 CAR-T cells (Extended Data Fig. 6g,h). Here, CD19 is linked to neoplasm.