IL2RG and Schnyder corneal dystrophy: To determine whether prime-edited HSPCs from SCD donors can repopulate bone marrow in vivo, we thawed cryopreserved prime-edited and untreated HSPCs from four SCD donors, then transplanted each via tail-vein injection into 2–5 immunodeficient NOD B6.SCID Il2rγ−/−KitW41/W41 (NBSGW) mice, which were pretreated with low-dose busulfan at 2 d before injection to enhance donor HSPC engraftment36 (Fig. 3a).