A prior study showed that different types of tumor cells induced necroptosis in endothelial cells to promote intravasation, but RCC models were not investigated.45 Co-culturing with RC-VHL-WT or RC-VHL-KO cells with HUVEC cells did not activate the necroptosis markers MLKL or RIP in HUVECs (Fig. 6a, b). Here, VHL is linked to neoplasm.