Unexpectedly, in PWS INS-1 cells there was a striking deficiency of multiple ER chaperone proteins, including GRP78/BiP (HSPA5), GRP94/endoplasmin (HSP90B1), PDIA4, HYOU1, CRELD2, and DNAJB11, with lesser reductions in SDF2L1, DNAJC3, PDIA6, and PDIA3, and a modest decrease in PPIB (Fig 3B). This evidence concerns the gene HSPA5 and Prader-Willi syndrome.