According to a study on the anticancer activity of the spontaneous nucleus-targeting core PF33/hTRAIL, HAC/hTRAIL, and RRPHC/hTRAIL complexes on HCT116 colorectal cancer cells, the TRAIL protein was significantly more highly expressed in the groups treated with PF33/hTRAIL and RRPHC/hTRAIL than in the groups treated with HAC/hTRAIL. The gene discussed is TNFSF10; the disease is colorectal cancer.