One meta-analysis showed that osteomyelitis (OR: 4.5), neuro-ischemic DFI (OR: 3.06), severe infection (OR: 3.12), leukocytosis (OR: 1.76), mean ESR (SMD: 0.5), mean CRP (SMD: 0.8), tissue culture positivity (OR: 1.61), and isolation of Gram-negative bacteria from tissue culture (OR: 1.5) were predictors of amputation in DFI (19). This evidence concerns the gene CRP and infection.