These results illustrated up-regulation of CAFs genes could play a precondition role in activating specific signaling pathways such as angiogenesis, epithelial mesenchymal transition, coagulation, hypoxia, and uv-response-down, etc. This alternation influenced the tumor microenvironment and leaded to poorer prognosis in head and neck squamous cell carcinoma patients. This evidence concerns the gene TBX1 and neoplasm.