In this study, HCT-116 and Caco-2 colon cancer cell lines and western blotting were used to investigate several proteins (i.e., Bax, Bcl-2, p53, CCND1, and c-Myc) that play a central role in cell apoptosis, proliferation, and cell cycle progression. The gene discussed is BAX; the disease is malignant colon neoplasm.