QRT-PCR and Western blot were used to detect CD86 expression in SKM-1 (myelodysplastic syndrome), OCI-AML2 (human myeloid leukemia cell), SH-1 (human myeloid leukemia cell), HL-60 (human myeloid leukemia cell), MEG01 (human megakaryoblastic leukemia cell), and K562 cells (human myeloid leukemia cell). This evidence concerns the gene CD86 and adult acute megakaryoblastic leukemia.